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Bacterial diversity and community structure of denitrifying phosphorus removal sludge in strict anaerobic/anoxic systems operated with different carbon sources
Author(s) -
Lv Xiaomei,
Shao Mingfei,
Li Chaolin,
Li Ji,
Xia Xue,
Liu Dongyang
Publication year - 2014
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4265
Subject(s) - denitrifying bacteria , anoxic waters , enhanced biological phosphorus removal , chemistry , phosphorus , propionate , glycerol , environmental chemistry , sequencing batch reactor , microbial population biology , pulp and paper industry , effluent , sewage treatment , activated sludge , denitrification , bacteria , biology , environmental engineering , biochemistry , organic chemistry , environmental science , nitrogen , engineering , genetics
BACKGROUND The effect of carbon source on the community structure of denitrifying phosphorus removal sludge has not yet been well established. In the current study, denitrifying phosphorus removal sludge with acetate, propionate and glycerol as the sole carbon source were acclimatized in three sequencing batch reactors ( SBRs ) under strict anaerobic‐anoxic conditions, and sludge community structure was systematically investigated by high‐throughput sequencing. RESULTS Average effluent phosphorus concentrations were 0.79 mg L −1 , 0.37 mg L −1 and 0.99 mg L −1 for SBR fed with acetate, propionate and glycerol, corresponding to phosphorus removals of 82.5%, 91.8% and 79.2%, respectively. Seed sludge from a wastewater treatment plant had the richest microbial diversity, followed by glycerol based sludge; acetate and propionate based sludge exhibited similar diversity and community structure. From acclimation to stable period, Ca. Accumulibacter ‐like sequences increased from 4.54%, 4.38% and 1.57% to 9.53%, 41.5% and 7.96% for sludge with acetate, propionate and glycerol as carbon source. For glycogen accumulating organism ( GAO )‐like sequences, it varied from 1.54%, 2.22% and 1.18% to 6.92%, 1.18% and 1.79%, respectively. CONCLUSIONS Propionate was a superior substrate favorable to the enrichment of phosphorus removal bacteria and elimination of glycogen accumulating bacteria in an anaerobic–anoxic system. Glycerol, a by‐product of biodiesel, could be an alternative carbon source for denitrifying phosphorus removal. © 2013 Society of Chemical Industry