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Microwave‐assisted aqueous two‐phase extraction of phenolics from grape ( Vitis vinifera ) seed
Author(s) -
Dang YanYan,
Zhang Hua,
Xiu ZhiLong
Publication year - 2014
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4241
Subject(s) - chemistry , abts , extraction (chemistry) , acetone , dpph , flavonoid , aqueous solution , aqueous two phase system , solvent , proanthocyanidin , chromatography , phenols , antioxidant , polyphenol , organic chemistry
BACKGROUND The wine making process produces a large quantity of grape marc, which includes grape seeds, skins, and stems. These byproducts comprise approximately 20% of the harvested grapes. Aqueous two‐phase extraction ( ATPE ) composed of short‐chain alcohol, inorganic salt, and water is a novel approach used to separate active constituents from fermentation broth or natural plant. A microwave‐assisted aqueous two‐phase extraction ( MAATPE ) method was used to obtain phenolic compounds from grape seeds. The partitioning behavior of the phenolic compounds in the MAATPE system was assessed. RESULTS Phenolic compounds were extracted into the top acetone‐rich phase at 32% (w/w) acetone/16% (w/w) ammonium citrate. The maximum yield of total phenolics, flavonoid, and proanthocyanidin were 82.7, 52.6, and 30.7 mg g −1 , respectively. The corresponding recovery rates were 97.1%, 97.9%, and 99.3%. The antioxidant activity of seed extracts was determined by 2,2‐diphenyl‐1‐picrylhydrazyl ( DPPH ) and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonate) ( ABTS ) radical‐scavenging ability ( IC 50 ). No significant correlation was found between free radical scavenging capacity and TP content. CONCLUSION MAATPE required lower solvent concentration and less treatment time to extract high amounts of phenolics from grape seeds compared with the other methods. MAATPE has the potential for the extraction, clarification, and concentration of target products using a single step. © 2013 Society of Chemical Industry