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Enzymatic activity of Cydia pomonella larvae reared on diets produced with agro‐industrial hydrolyzed wastes
Author(s) -
Gnepe Jean Robert,
Tyagi Rajeshwar Dayal,
Brar Satinder Kaur,
Valéro José R.,
Surampalli Rao Y.
Publication year - 2013
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.4056
Subject(s) - nutrient , pomace , codling moth , food science , amylase , lipase , chemistry , enzymatic hydrolysis , hydrolysis , biology , larva , zoology , botany , enzyme , biochemistry , ecology
BACKGROUND This study compared the physical‐chemical characteristics and the nutrient potential of brewery wastewater ( BWW ) and apple juice industry sludge referred to as pomace ( POM ). The use of these wastes as alternative nutrients by replacing the standard components (soya flour, wheat germ and yeast extract) allowed production of a balanced diet for rearing codling moth ( CM ) larvae.RESULTS The hydrolysis pretreatment (100 °C/30 min) contributed to enhancing the nutritive potential (almost 600%) and improved the viscosity (decreased from 770.5 to 435 mPa s for BWW and from 460 to 383 mPa s for POM ) of the waste. The BWWH + SF diet supplied higher assimilated nutrients (2.071 mg proteins, 2.274 mg carbohydrates and 0.974 mg lipids) showing higher breeding efficiency (93% hatching, 85% larvae, 76% cocoon and 70% adults). Furthermore, the results of the larvae breeding on BWWH + SF diet was the best which for the enzymatic activity (59.7 UI mL ‐1 protease, 2.9 mg L −1 amylase and 1.93 U mg −1 lipase), consumption rates of proteins (451.79 mg kg −1 ), carbohydrates (396.43 mg kg −1 ) and lipids (91.07 mg kg −1 ) consumed on these diets.CONCLUSIONS BWWH + SF was the best diet with respect to enzymatic activity, consumption of nutrients and larval breeding efficiency compared with POMH + SF and the standard diet. © 2013 Society of Chemical Industry