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A novel process for the recovery of superoxide dismutase from yeast exploiting electroextraction coupled to direct sorption
Author(s) -
SimentalMartínez Jesús,
Vennapusa Rami Reddy,
Benavides Jorge,
Ganeva Valentina,
Galutzov Bojidar,
RitoPalomares Marco,
FernándezLahore Marcelo
Publication year - 2013
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.3993
Subject(s) - kluyveromyces marxianus , amberlite , chemistry , chromatography , ion exchange , ion chromatography , yeast , superoxide dismutase , kluyveromyces , yield (engineering) , adsorption , enzyme , ion exchange resin , biochemistry , organic chemistry , ion , materials science , saccharomyces cerevisiae , metallurgy
Abstract Background This work presents a novel approach for the recovery and purification of superoxide dismutase ( SOD ; EC 1.15.1.1) produced in the yeast Kluyveromyces marxianus var. bulgaricus ( NBIMCC 1984). Electroextraction was evaluated as an alternative to bead milling for SOD release. Seven anion exchange resins were studied in order to optimize SOD purification using ion exchange chromatography . Results Electroextraction produced an enzyme solution with a specific activity 13 times higher than with mechanical extraction, demonstrating the potential of this novel technique for the selective liberation of SOD . Regarding the anion exchange resins that were studied, Amberlite™ IRA ‐96 and Q Sepharose™ XL were chosen as the best options for purifying SOD using chromatography in negative adsorption mode. The process developed, involving electroextraction, enzyme concentration and negative anion exchange chromatography, rendered a highly active and pure SOD preparation (specific activity: 2056 U mg −1 and 66‐fold purification) with an overall recovery yield of 84% . Conclusion The results presented herein demonstrate the potential of the process developed for the recovery and purification of SOD from the yeast Kluyveromyces marxianus var. bulgaricus ( NBIMCC 1984). The preparation obtained could be used in the food industry as an antioxidant agent. © 2013 Society of Chemical Industry