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Supercritical and enzymatic technologies for the production of lysophosphatidylcholine
Author(s) -
Casado Víctor,
Señorans Francisco.J,
Reglero Guillermo,
Torres Carlos F.
Publication year - 2013
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.3842
Subject(s) - phosphatidylcholine , lysophosphatidylcholine , chemistry , formic acid , supercritical fluid , hydrolysis , chromatography , extraction (chemistry) , supercritical carbon dioxide , methanol , solvent , organic chemistry , phospholipid , biochemistry , membrane
BACKGROUND This study focuses on obtaining a highly purified lysophosphatidylcholine from phosphatidylcholine powder via hydrolysis, catalyzed by a phospholipase A2 in a solvent‐free medium, and subsequent purification either by organic solvent or by supercritical fluid extraction.RESULTS A solvent‐free hydrolysis reaction of phosphatidylcholine powder to produce lysophosphatidylcholine with residual phosphatidylcholine content lower than 10% has been described and scaled‐up. Supercritical fluid mixtures of carbon dioxide, ethanol and formic acid were studied at 250, bar 50 °C to selectively extract the free fatty acids fraction from hydrolyzed phosphatidylcholine. Finally, a solid product with lysophosphatidylcholine content of c . 95% (w/w) and with free fatty acids content of c . 5% (w/w) was attained. Under optimum conditions, the content of the original phosphatidylcholine in the lysophosphatidylcholine product was lower than 5% (w/w).CONCLUSIONS An efficient hydrolysis of phosphatidylcholine by phospholipase A2 in a solvent‐free reaction was developed. In addition, three novel strategies for the purification of lysophosphatidylcholine have been developed: (1) hexane/formic acid extraction process, (2) supercritical CO 2 extraction with ethanol as modifier, and (3) supercritical CO 2 extraction with ethanol/formic acid as modifier. Copyright © 2012 Society of Chemical Industry