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Studies on the interaction of CdTe QDs with bovine serum albumin
Author(s) -
Liu Peng,
Liu Yisi,
Wang Qisui
Publication year - 2012
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.3815
Subject(s) - bovine serum albumin , circular dichroism , ionic strength , quenching (fluorescence) , chemistry , fluorescence , quantum dot , ionic bonding , hydrophobic effect , analytical chemistry (journal) , crystallography , nanotechnology , chromatography , materials science , biochemistry , aqueous solution , organic chemistry , ion , physics , quantum mechanics
BACKGROUND: Quantum dots (QDs) have attracted much attention in biological and medical applications. In particular, the interaction of QDs with bovine serum albumin (BSA) is crucial, and has been systematically investigated by various spectroscopic techniques under the physiological conditions. RESULTS: The effects of ionic strength and pH on the interaction of CdTe QDs with BSA were studied by changing NaCl concentration and pH in mixed solution and making fluorescence spectroscopic measurements. The Stern‐Volmer quenching constant ( K a ) of different ionic strength and pH were calculated, and information on the structural features of BSA were discussed by means of circular dichroism (CD) spectrum. CONCLUSION: Both fluorescence (FL) and circular dichroism (CD) results indicated that hydrophobic and electrostatic interactions play a major role in the binding reaction, and the nature of quenching is static, resulting in forming QDs‐BSA complexes. Copyright © 2012 Society of Chemical Industry