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Fabrication and characterization of albumin‐acacia nanoparticles based on complex coacervation as potent nanocarrier
Author(s) -
Hedayati Rouhollah,
Jahanshahi Mohsen,
Attar Hussain
Publication year - 2012
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.3758
Subject(s) - coacervate , biopolymer , nanocarriers , ionic strength , tragacanth , bovine serum albumin , nanoparticle , dispersity , chemical engineering , glutaraldehyde , materials science , chemistry , nuclear chemistry , chromatography , nanotechnology , polymer chemistry , organic chemistry , polymer , food science , aqueous solution , engineering
Abstract BACKGROUND: Bovine serum albumin (BSA) and Gum Arabic (Acacia) were used to fabricate nanoparticles (NPs) as a new nanocarrier for drug and bioactive delivery systems. Several parameters, namely pH, protein to polysaccharide ratio, ionic strength, total biopolymer concentration, temperature, agitation speed and glutaraldehyde concentration were investigated for their effect on the product preparation process. Besides fabrication, the stabilization step including heating, ultrasonicating, cross‐linking, and its effect on average size and polydispersity of NPs was examined. RESULTS: Total biopolymer concentration had no marked effect on the average size of prepared NPs although it did affect other systems produced by complex coacervation. The most effective parameters were pH, temperature and ionic strength, which affected this system (albumin‐acacia) by a complicated route. The smallest size of prepared NPs (i.e. the best product) was 108 nm, which was fabricated under the optimum condition of T = 4 °C, pH = 4.9, $Pr{:}Ps = 2$ , and Is = 0. CONCLUSION: Of the stabilization methods tested, heating gave the best results. The fabricated NPs were purified with a 0.45 µm syringe filter and analyzed by PCS, SEM, FTIR and DSC. The mechanism of complex coacervation for preparing NPs, and their use as a nanodelivery system are discussed. Copyright © 2012 Society of Chemical Industry