Premium
Optimization of biological synthesis of silver nanoparticles using Lactobacillus casei subsp. casei
Author(s) -
Korbekandi Hassan,
Iravani Siavash,
Abbasi Sajjad
Publication year - 2012
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.3702
Subject(s) - lactobacillus casei , silver nanoparticle , nanoparticle , biotransformation , substrate (aquarium) , inducer , yield (engineering) , chemistry , nuclear chemistry , enzyme , nanotechnology , materials science , fermentation , biochemistry , biology , ecology , gene , metallurgy
BACKGROUND: The objectives of this study were optimization of silver nanoparticle synthesis using biotransformations by Lactobacillus casei subsp. casei, and studying the location of nanoparticles synthesis in this microorganism. RESULTS: The presence of AgNO 3 (0.1 mmol L −1 ) in the culture as the enzyme inducer, and glucose (56 mmol L −1 ) as the electron donor in the reaction mixture had positive effects on nanoparticle production. By gradually increasing the concentration of AgNO 3 (as the substrate) to 6 mmol L −1 , nanoparticle production was increased. By increasing biomass, nanoparticles production was also increased. Biosynthesized silver nanoparticles were almost spherical, single (25–50 nm) or in aggregates (100 nm), attached to the surface of biomass or were inside and outside of the cells. CONCLUSION: The present study demonstrated the bioreductive synthesis of silver nanoparticles using L. casei subsp. casei at room temperature. In this research, and due to experience in optimization of biotransformation reactions, the reaction conditions were successfully optimized to increase the yield of nanoparticles production and productivity of this biosynthetic approach. Copyright © 2012 Society of Chemical Industry