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Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes
Author(s) -
Enman Josefine,
Hodge David,
Berglund Kris Arvid,
Rova Ulrika
Publication year - 2012
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.3697
Subject(s) - lentinus , mycelium , mushroom , food science , bioreactor , chemistry , biomass (ecology) , edible mushroom , horticulture , biology , agronomy , organic chemistry
BACKGROUND: Mycelium of the medicinal mushroom shiitake, Lentinus edodes , is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. RESULTS: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2–3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L −1 . Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L −1 , which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. CONCLUSION: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production. Copyright © 2012 Society of Chemical Industry