Premium
Use of a genetic algorithm in the development of a synthetic growth medium for Arthrobacter simplex with high hydrocortisone Δ 1 ‐dehydrogenase activity
Author(s) -
WeusterBotz D.,
Pramatarova V.,
Spassov G.,
Wandrey C.
Publication year - 1995
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280640411
Subject(s) - dehydrogenase , simplex , biomass (ecology) , hydrocortisone , chemistry , yield (engineering) , arthrobacter , biochemistry , biology , mathematics , enzyme , physics , thermodynamics , combinatorics , ecology
The production process for Arthrobacter simplex high specific hydrocortisone Δ 1 ‐dehydrogenase activity and biomass yield (full experimental plan: 1·5 × 10 14 experiments). The specific hydrocortisone Δ 1 ‐dehydrogenase activity was improved by a vector of 4·5 to 1800 U g −1 . With the optimized medium composition in a stirred tank reactor for batch production of A. simplex , a specific hydrocortisone Δ 1 ‐dehydrogenase activity of 2000–3000 U g −1 dry biomass was achieved depending on harvest time (previously reported data: 18–37 U g −1 ).