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Production of L ‐aminoacylase by fermentation of Pseudomonas sp. BA2
Author(s) -
Santoyo Antonio Bódalo,
Rodríguez Josefa Bastida,
Carrasco José Luis Gómez,
Gómez Elisa Gómez,
Teruel María Luisa Asanza,
Rojo Isabel Alcaraz
Publication year - 1995
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280640210
Subject(s) - industrial fermentation , fermentation , chemistry , food science , pseudomonas , substrate (aquarium) , dry weight , biomass (ecology) , microorganism , bacterial growth , nitrogen , bacteria , biology , botany , agronomy , organic chemistry , ecology , genetics
Abstract The growth and enzymatic production of Pseudomonas sp. BA2 a new L ‐aminoacylase‐producing microorganism, were studied in a bench‐top fermenter. Multiple fermentations were carried out in order to determine the optical pH and temperature values. The influence of the substrate concentration on both growth and L aminoacylase activity was also investigated. The maximum growth rate and the greatest yield of enzyme were obtained when the fermentation was carried out at pH 7·5, 25°C and DOT ≥ 50%. N ‐Acetyl‐ DL ‐alanine, at a concentration 20 g dm −3 , was used as the sole carbon and nitrogen source. The fermentation process provided a maximum biomass concentration of 3·36 g dry weight dm −3 . The highest L ‐aminoacylase production (11429 U g −1 dry weight) was obtained after 39 h of cultivation. The results were a significant improvement over those previously reported.