Production of L ‐aminoacylase by fermentation of Pseudomonas sp. BA2

The growth and enzymatic production of Pseudomonas sp. BA2 a new L ‐aminoacylase‐producing microorganism, were studied in a bench‐top fermenter. Multiple fermentations were carried out in order to determine the optical pH and temperature values. The influence of the substrate concentration on both growth and L aminoacylase activity was also investigated. The maximum growth rate and the greatest yield of enzyme were obtained when the fermentation was carried out at pH 7·5, 25°C and DOT ≥ 50%. N ‐Acetyl‐ DL ‐alanine, at a concentration 20 g dm −3 , was used as the sole carbon and nitrogen source. The fermentation process provided a maximum biomass concentration of 3·36 g dry weight dm −3 . The highest L ‐aminoacylase production (11429 U g −1 dry weight) was obtained after 39 h of cultivation. The results were a significant improvement over those previously reported.