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Micromixing and the steady‐state performance of bioreactors using recombinant bacteria—analysis through a reversed two‐environment model
Author(s) -
Patnaik Pratap R.
Publication year - 1994
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280610409
Subject(s) - micromixing , dilution , bacteria , bioreactor , steady state (chemistry) , chromatography , chemistry , escherichia coli , substrate (aquarium) , fermentation , plasmid , recombinant dna , biology , biochemistry , analytical chemistry (journal) , thermodynamics , organic chemistry , dna , ecology , physics , genetics , gene
A reversed two‐environment model has been to study micromixing in continuous fermentation for growth and product formation by recombinant bacteria. As an example, an Escherichia coli M72 strain harbouring the plasmid pPLc23trpA1 and producing tryptophan synthetase is considered. With excess substrate, 10% plasmid‐free bacteria in the initial broth do not affect the steady‐state concentrations of plasmid‐containing bacteria but their mass fractions decrease significantly. With a smaller substrate concentration, the mass fractions decrease sharply as the dilution rate increases if micromixing is good; but concentrations still remain high. There is also a clear demarcation between regions of good and poor micromixing. These results are explained and an optimal combination of micromixing and dilution rate is suggested to maximise productivity.