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A flow injection system for simultaneous determination of starch and glucose concentrations
Author(s) -
Umoh Enobong F.,
Schügerl Karl
Publication year - 1994
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280610112
Subject(s) - glucose oxidase , chemistry , chromatography , starch , flow injection analysis , amylase , glucose syrup , sugar , linear range , detection limit , biosensor , biochemistry , enzyme
A flow injection analysis (FIA) system capable of simultaneously determining the concentrations of starch and glucose in samples was set up. The system consisted of glucoamylase (GA), glucose oxidase (GOD), (EC 1.1.3.4) and mutarotase (MUT, EC 5.1.3.3) immobilized on macroporous polymer packed in reaction cartridges was operated at 30°C. Glucose resulting from the action of glycoamylase or originally present in the samples was determined amperometrically. The starch was analysed with the system without needing prehydrolysis with α‐amylase, and inclusion of mutarotase was found necessary only when the sample originally contained α‐glucose. A linear detection range up to 1.5 g dm −3 and 4.0 g dm −3 was obtained for the streams from which glucose and total sugar concentrations were measured, respectively. The system was able to analyse up to 10 samples per hour.