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Stability of a mineral membrane ultrafiltration reactor for peptide hydrolysis of hemoglobin
Author(s) -
Sannier Frédéric,
Piot JeanMarie,
Dhulster Pascal,
Guillochon Didier
Publication year - 1994
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280610107
Subject(s) - pepsin , ultrafiltration (renal) , chemistry , hemoglobin , hydrolysis , chromatography , autolysis (biology) , membrane , peptide , permeation , biochemistry , enzyme
Factors involved in the activity decay of an ultrafiltration reactor during the hydrolysis of hemoglobin by pepsin were studied. Biochemical and hydrodynamic parameters were assessed. The product of hydrolysis showed no inhibitory effect. Autolysis and mechanical damage of pepsin were limited by the presence of hemoglobin. Activity decay was mainly a result of the leakage of pepsin through the membrane. Electrostatic charges carried by either hemoglobin or pepsin were involved in the permeation process.