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Kinetic analysis of benzyl alcohol oxidation by alcohol oxidase in aqueous—organic two‐phase systems
Author(s) -
Mitarai Hitomi,
Kawakami Koei
Publication year - 1994
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280600407
Subject(s) - benzaldehyde , benzyl alcohol , chemistry , aqueous two phase system , aqueous solution , decane , organic chemistry , aldehyde , alcohol , substrate (aquarium) , solvent , reagent , ethyl acetate , catalysis , oceanography , geology
Alcohol oxidase‐catalyzed oxidation of benzyl alcohol to benzaldehyde was studied in three two‐liquid phase systems involving butyl acetate, xylene and n ‐decane. A more highly hydrophobic solvent resulted in more favorable partitioning of the substrate into the aqueous phase and less deleterious effect on the enzyme activity. As a result, the overall production of aldehyde was highest with the n ‐decane system and lowest with the butyl acetate system. In addition, the organic solvent served as an extractant for the product, which acted both as a competitive inhibitor and as a denaturant of the enzyme. The time courses of benzaldehyde production were simulated by a simple mathematical model assuming Michaelis–Menten kinetics in the aqueous phase, enzyme deactivation by organic solvents and the equilibrium partitioning of the reagents between the two phases.