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Immobilisation of β‐galactosidase onto gelatin by glutaraldehyde and chromium(III) acetate
Author(s) -
Sungur Sibel,
Akbulut Ural
Publication year - 1994
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280590314
Subject(s) - glutaraldehyde , gelatin , chemistry , chromium , enzyme , chromatography , polymer chemistry , nuclear chemistry , biochemistry , organic chemistry
β‐Galactosidase (EC 3.2.1.23) was immobilised onto a gelatin carrier system by crosslinking. Glutaraldehyde and chromium(III) acetate were used as crosslinking agents. The effect of pH on enzyme activity, kinetic parameters and reusability of the immobilised enzyme were investigated. To obtain a better relative activity, a method was developed and further modified to increase the surface area of the support system by using polyacrylamide. Relative activity enhancement of 68% was achieved in the gelatin–glutaraldehyde system.