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A lysine sensor for process control
Author(s) -
Siegler Klaus,
Weber Bettina,
Weber Ekkehard,
Tonder Karin,
Klingner Edith,
Riechert Frank
Publication year - 1994
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280590311
Subject(s) - lysine , trichoderma viride , chemistry , polyurethane , cellulose , electrode , amino acid , biochemistry , food science , organic chemistry
A lysine sensor for process control of lysine fermentation was developed based on a Clark‐type electrode in combination with L ‐lysine‐α‐oxidase. The enzyme, isolated from Trichoderma viride , was immobilized between a cellulose and a polypropylene foil using a polyurethane resin. Lysine determinations were carried out in a flow‐through system as anodic measurements when H 2 O 2 was measured and as chathodic measurements when the consumption of O 2 was followed. The sensitivity of the sensor toward other amino acids was determined.