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Application of five‐state structured models for growth and secondary metabolite production in cultures of Nicotiana tabacum
Author(s) -
Shibasaki Naomi,
Yonemoto Toshikuni,
Tadaki Teiriki
Publication year - 1993
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280580208
Subject(s) - nicotiana tabacum , catharanthus roseus , vacuole , protoplast , bioreactor , metabolite , secondary metabolite , suspension culture , biology , botany , airlift , cell culture , plant cell , biological system , microbiology and biotechnology , biochemistry , genetics , cytoplasm , gene
Abstract A new structured model was constructed and applied to batch suspension cultures of Nicotiana tabacum in an airlift bioreactor with a draught tube. This model was developed by modifying the structured model proposed by Bailey and Nicholson 1,2 for cultures of Catharanthus roseus cells. In this model, a plant cell was considered to consist of a vacuole and non‐vacuole. A secondary metabolite was also assumed to be synthesized by viable cells and stored at a constant concentration in the vacuole. Parameters for the model equations were estimated based upon experimental data using numerical optimization techniques. The simulated results using these parameters showed that the present model was superior to the earlier model for describing growth and product synthesis during all culture periods for the tobacco cell cultures.