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Constant flowrate filtration of hybridoma cells suspensions
Author(s) -
Favre Eric
Publication year - 1993
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280580202
Subject(s) - pressure drop , filtration (mathematics) , fouling , volumetric flow rate , cross flow filtration , drop (telecommunication) , constant (computer programming) , compressibility , chemistry , mechanics , chromatography , materials science , environmental engineering , membrane , environmental science , mathematics , physics , engineering , mechanical engineering , biochemistry , statistics , computer science , programming language
The fouling mechanism of rotating sieves (also called spinfilters) in suspended mammalian cells reactors is still poorly understood, and their design is consequently largely empirical. A laboratory set‐up enabling constant flowrate filtration experiments is reported and its use described in order to study the fouling kinetics of 10 μm opening sieves by suspended hybridomas. Significant differences were obtained depending upon cell type and cell viability, under similar operating conditions. A roughly linear pressure drop increase, as predicted by constant flowrate incompressible cake filtration models, was obtained for most experiments. However, the influences of pumping flowrate and cell concentration on pressure drop evolution do not obey incompressible cake constant flowrate filtration laws; a surface colonization approach could possibly lead to a better description in that case.