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Immobilization of penicillin acylase on copolymer of butyl acrylate and ethylene glycol dimethacrylate
Author(s) -
Bryjak Jolanta,
Noworyta Andrzej
Publication year - 1993
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280570114
Subject(s) - glutaraldehyde , immobilized enzyme , hydrolysis , chemistry , ionic strength , penicillin amidase , copolymer , butyl acrylate , acrylate , ethylene glycol , chromatography , polymer chemistry , nuclear chemistry , organic chemistry , enzyme , polymer , aqueous solution
The effects of glutaraldehyde, enzyme concentrations and reactants volumes, ionic strength, pH value and carrier particle diameter on immobilization of penicillin acylase onto acrylic carriers were studied. The activity of immobilized enzyme preparations was also studied over a range of pH values and temperatures and thermal and pH stabilities were determined. The use of the immobilized preparation for penicillin G hydrolysis in a batch reactor was investigated. The immobilized enzyme gave a significant reduction in hydrolysis time compared to hydrolysis by the native enzyme.