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Maximizing the production of acetone—butanol in an alginate bead fluidized bed reactor using clostridium acetobutylicum
Author(s) -
Mollah Abdul H.,
Stuckey David C.
Publication year - 1993
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280560115
Subject(s) - clostridium acetobutylicum , acetone , butanol , dilution , chemistry , fermentation , chromatography , bioreactor , solvent , fluidized bed , calcium alginate , aqueous two phase system , aqueous solution , biochemistry , ethanol , calcium , organic chemistry , physics , thermodynamics
Low volumetric solvent productivities are one of the characteristics of an acetone‐butanol fermentation by C. acetobutylicum. A calcium alginate immobilized continuous culture was used in a novel gas‐sparged reactor to strip the solvents from the aqueous phase and reduce their toxicity. A dilution rate of 0.07 h −1 was found to give maximum solvent productivity at 0.58 g dm −3 h −1 , although at 0.12 h −1 the productivity was slightly lower. In order to increase glucose uptake by the culture, feed glucose concentrations were increased over time to attempt to acclimatize the culture. This resulted in a productivity as high as 0.72 g dm −3 h −1 although this production rate was found to be unstable.