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Production of the enzyme dihydrofolate reductase by methotrexate‐resistant bacteria isolated from soil
Author(s) -
Nigam Poonam,
Marchant Roger
Publication year - 1993
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280560107
Subject(s) - dihydrofolate reductase , methotrexate , enzyme , bacteria , microbiology and biotechnology , biochemistry , chemistry , biology , genetics , immunology
Six bacterial cultures isolated from soil were capable of growing in the presence of methotrexate (MTX). Two strains, PFR‐1 and 3, developed resistance to 500 μg cm −3 MTX in the medium and produced elevated levels of the enzyme dihydrofolate reductase (EC 1.5.1.3): 2580 and 2702 U dm −3 compared to the sensitive parent strains (28 and 35 U dm −3 ). Isolate PFR‐3 showed maximum enzyme production (4950 U dm −3 , specific activity 12.56 U mg −1 in flasks and 5737 U dm −3 , specific activity 14.80 U mg −1 in 5‐dm 3 fermenter) during exponential phase of growth (6 h) at 37°C and pH 7.0.