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A comparative study of the performance of solid supported and soluble urease for the enzymatic hydrolysis of urea
Author(s) -
Demirel Günay,
Akovali GÜNeri,
Tanyolac Abdurrahman,
Hasirci Nesrin
Publication year - 1992
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280550403
Subject(s) - urease , urea , hydrolysis , chemistry , enzyme , enzymatic hydrolysis , kinetics , detoxification (alternative medicine) , chromatography , organic chemistry , medicine , physics , alternative medicine , quantum mechanics , pathology
The performance of both free and solid supported urease for the enzymatic hydrolysis of urea was studied. Kinetic analysis of reaction rate data shows that the kinetic data were consistent with the proposed model. Statistical tests validated the model. Despite the deactivation of ureases, the immobilized enzyme could be the choice for a technological process aiming at the detoxification of blood in haemoperfusion columns.