Premium
The application of aqueous two‐phase systems to oligosaccharide synthesis by a‐mannosidase catalysed glycosyl transfer reactions
Author(s) -
Bartlett Terence J.,
Rastall Robert A.,
Rees Nicholas H.,
Adlard Maxwell W.,
Bucke Christopher
Publication year - 1992
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280550112
Subject(s) - oligosaccharide , chemistry , yield (engineering) , aqueous solution , glycosyl , phase (matter) , aqueous two phase system , dextran , mannosidase , peg ratio , combinatorial chemistry , chromatography , organic chemistry , enzyme , materials science , metallurgy , finance , economics
Aqueous two‐phase systems formed from PEG and dextran have been applied to the synthesis of oligosaccharide by Jack bean α‐mannosidase in reverse. Whilst rates of synthesis and percentage yields weie similar in two‐phase systems and one‐phase aqueous buffer systems, a ten‐fold increase in yield of product per unit of enzyme was seen. In addition, the use of aqueous two‐phase systems offers potential process advantages over one‐phase systems for the synthesis of oligosaccharides.