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Permeation of 6‐nitro‐3‐phenylacetamide benzoic acid (nipab) and hydrolysis by penicillin acylase immobilized in emulsion liquid membranes
Author(s) -
Miesiac I.,
Schügerl K.,
Szymanowski J.
Publication year - 1992
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280550102
Subject(s) - permeation , chemistry , emulsion , benzoic acid , membrane , chromatography , hydrolysis , potassium , organic chemistry , chemical engineering , biochemistry , engineering
The effects of various commercial and model surfactants of different structure and hydrophilicity were studied on water‐in‐oil (w/o) emulsion stability, potassium cation leakage and permeation of 6‐nitro‐3‐phenylacetamide benzoic acid in a model system using Penicillin acylase (EC 3.5.1.11) immobilized in a liquid membrane. Both emulsion stability, potassium leakage and permeation of organic substances depend upon hydrophilicity; of surfactants. Hydrophilic surfactants may be used to stabilize emulsions only in mixtures with hydrophobic emulsifiers. Additions of small quantities of hydrophilic surfactants to the system in which permeation occurs together within an enzymatic process may be advantageous. Both the rate of permeation and potassium transfer significantly increase when hydrophilic surfactants are present. There was no relationship observed between potassium cation transfer from the internal phase and emulsion stability in the storage lest.