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Kinetic study of an enzymatic liquid—liquid reaction: The hydrolysis of tributyrin by Candida cylindracea lipase
Author(s) -
Martinez Oswaldo,
Wilhelm AnneMarie,
Riba JeanPierre
Publication year - 1992
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280530409
Subject(s) - tributyrin , lipase , chemistry , hydrolysis , chromatography , reaction rate , kinetic energy , thermodynamics , enzyme , organic chemistry , catalysis , physics , quantum mechanics
The objective of the work was to derive a kinetic model for the enzymatic hydrolysis of oils. The model reaction examined was the lipolysis of tributyrin by Candida cylindracea lipase. The kinetic model of Verger et al 14 was adapted to the particular case of a standard, mechanically‐stirred tank in order to derive the reaction rate as a function of the variables ø, volume fraction of dispersed phase and N , speed of agitation. The validity of a literature correlation relating the mean drop diameter to ø, N and the interfacial tension was verified experimentally. The kinetic relationship was validated by a Lineweaver—Burk representation of the initial reaction rates determined and the kinetic constants were identified. For the system examined, the influence of stirrer speed was weak and limited to low values of ø (ø < 10%).