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Characterization and use of a penicillin acylase biocatalyst
Author(s) -
Ospina S. Sonia,
LopezMunguia Agustín,
Gonzalez Rosa Luz,
Quintero Rodolfo
Publication year - 1992
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280530217
Subject(s) - biocatalysis , arrhenius equation , chemistry , stability (learning theory) , continuous stirred tank reactor , characterization (materials science) , thermodynamics , kinetic energy , chemical engineering , chromatography , biochemical engineering , materials science , catalysis , organic chemistry , computer science , nanotechnology , reaction mechanism , activation energy , physics , engineering , machine learning , quantum mechanics
A complete characterization of a penicillin acylase biocatalyst is presented, including the determination of physicochemical and kinetic parameters. Stability studies are detailed in terms of both storage temperature and pH as well as operational stability after 150 batch reactions of two hours duration each. An Arrhenius‐type model was used to simulate the effect of pH on biocatalyst stability. A kinetic model is proposed to describe batch and continuous stirred tank reactors and to predict the long‐term behavior of the process.