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A simple affinity chromatography method for the separation of gastric proteases from mucous substances
Author(s) -
Łobarzewski J.,
KowalskaPyłka H.,
Cybulski W.
Publication year - 1991
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280520308
Subject(s) - proteases , chromatography , elution , chemistry , keratin , matrix (chemical analysis) , affinity chromatography , ligand (biochemistry) , biochemistry , enzyme , biology , receptor , paleontology
A simple chromatographic method for separating mucous substances contained in the abomasal mucosa from proteases has been developed. The chromatographic technique used controlled porous glass as a matrix, with covalently bound keratin as a ligand. This matrix allowed affinity binding of proteases at pH 7·0. The mucous substances present in calf abomasal mucosa were easily identified in the column eluate. Proteases were eluted from the column with 0·1 ml dm −3 HCl with good recovery (85%) and high purification.