Premium
Stabilization‐activation of pancreatic enzymes adsorbed on to a sepiolite clay
Author(s) -
Cabezas M. J.,
Salvador D.,
Sinisterra J. V.
Publication year - 1991
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280520213
Subject(s) - sepiolite , adsorption , enzyme , pancreatic enzymes , chemistry , chemical engineering , clay minerals , materials science , biochemistry , mineralogy , organic chemistry , engineering , raw material
The addition of small quantities 1–2% (w/w) of the sepiolite clay EXAL to animal feed increases the feed efficiency and weight of animals. α‐Chymotrypsin, lipase and α‐amylase from bovine pancreas adsorbed on to EXAL at different pH values form active stable complexes at enteric pH values. The enzymes are chemisorbed on EXAL by interaction between NH 3 + groups of lysines and basic sites of the solid surface. The derivatives obtained are stable, regardless of changes in pH, and the relation between their activity and pH is complementary to that observed with the native enzyme. Therefore, these enzyme‐EXAL derivatives are resistant to proteolysis and increase the amount of active digestive enzymes in the intestine. A more efficient utilization of feed would take place in animals that have ingested EXAL than in those which have not ingested the sepiolite.