Premium
The recovery of fructose from inverted sugar beet molasses using continuous chromatography
Author(s) -
Barker Philip E.,
Joshi Ketan
Publication year - 1991
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280520107
Subject(s) - chromatography , fructose , chemistry , sugar beet , calcium , sugar , ion chromatography , ion exchange resin , biochemistry , organic chemistry , horticulture , biology
The continuous chromatographic separation of inverted beet molasses resulting in a fructose rich product and a product containing glucose and other non‐sugars was carried out using a semi‐continuous countercurrent chromatographic refiner (SCCR6), consisting of ten 10.8 cm diameter by 75 cm long stainless steel columns packed with a calcium charged 8% cross‐linked polystyrene resin, zerolit SRC14. It was found that cations present in beet molasses displaced calcium ions from the resin resulting in poor separation of the glucose and fructose. Three methods of maintaining the calcium form of the resin during the continuous operation of the equipment were established. Passing a solution of calcium nitrate through the purge column for half a switch period was found to be most effective as there was no contamination of the main fructose rich product and the product concentrations were increased by 50%.