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Bioconversion of L malic acid into L lactic acid using a high compacting multiphasic reactor (HCMR)
Author(s) -
Naouri Pascal,
Bernet Nicolas,
Chagnaud Patrice,
Arnaud Alain,
Galzy Pierre,
Rios Gilbert
Publication year - 1991
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280510109
Subject(s) - lactic acid , malolactic fermentation , leuconostoc , chemistry , malic acid , biotransformation , bioconversion , food science , lactobacillus , bacteria , biochemistry , nuclear chemistry , chromatography , citric acid , enzyme , fermentation , biology , genetics
Biotransformation of L malic acid into L lactic acid was studied in a fluidised bed reactor using cells of Lactobacillus sp. 89 and Leuconostoc oenos ATCC 23278 immobilised in a calcium alginate matrix. The reactor was used in a batch or a continuous manner under a non‐oxidising atmosphere. It was shown that 3 mm diameter beads loaded with 6 and 30 mg dry matter per gram of gel were the best operating conditions for Leuconostoc and Lactobacillus respectively. The optimal working conditions were as follows: pH = 3.0–5.0, temperature = 30°C for Lactobacillus and pH = 3.0, temperature = 25°C for Leuconostoc. No inhibition due to lactic acid was observed and the internal diffusional effects were negligible. The short catalyst life that was initially observed with both strains resulted from a depletion in NAD and Mn 2+ , two basic cofactors for malolactic enzymes. To prevent this detrimental effect, a regeneration process for biocatalyst was proposed.