Immobilization of  Desulfovibrio vulgaris  cells with hydrogenase activity | Zendy

Barreto M. C. | Zendy; Cabral J. M. S. | Zendy

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Immobilization of Desulfovibrio vulgaris cells with hydrogenase activity

Author(s) -

Barreto M. C.,

Cabral J. M. S.

Publication year - 1991

Publication title -

journal of chemical technology and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.64

H-Index - 117

eISSN - 1097-4660

pISSN - 0268-2575

DOI - 10.1002/jctb.280500413

Subject(s) - desulfovibrio vulgaris , hydrogenase , desulfovibrio , chemistry , calcium alginate , biochemistry , oxygen , calcium , enzyme , bacteria , nuclear chemistry , biology , organic chemistry , genetics , sulfate

Desulfovibrio vulgaris intact cells immobilized in calcium alginate retained 45% of the hydrogenase activity of free cells. Cell immobilization had no effect on the optimum temperature for the hydrogen uptake rate, whereas the pH optimum was shifted to a higher value. The immobilization conferred on the cells a protection both against oxygen inhibition and thermal denaturation of the hydrogenase system.

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