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Immobilization of Desulfovibrio vulgaris cells with hydrogenase activity
Author(s) -
Barreto M. C.,
Cabral J. M. S.
Publication year - 1991
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280500413
Subject(s) - desulfovibrio vulgaris , hydrogenase , desulfovibrio , chemistry , calcium alginate , biochemistry , oxygen , calcium , enzyme , bacteria , nuclear chemistry , biology , organic chemistry , genetics , sulfate
Desulfovibrio vulgaris intact cells immobilized in calcium alginate retained 45% of the hydrogenase activity of free cells. Cell immobilization had no effect on the optimum temperature for the hydrogen uptake rate, whereas the pH optimum was shifted to a higher value. The immobilization conferred on the cells a protection both against oxygen inhibition and thermal denaturation of the hydrogenase system.