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Recycling of salts in partition protein extraction processes
Author(s) -
Greve Arend,
Kula MariaRegina
Publication year - 1991
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280500105
Subject(s) - extraction (chemistry) , salt (chemistry) , chemistry , chromatography , microfiltration , nucleic acid , aqueous solution , pollution , phase (matter) , organic chemistry , membrane , biochemistry , biology , ecology
Aqueous two‐phase extraction systems were developed to separate intracellular proteins from cell debris. To economize on the use of chemicals as well as to minimize environmental pollution, a closed mode of operation was sought. Various approaches to achieve recycling of salt from the primary lower phase containing 30–60% (w/w) of the added salt together with cell debris, proteins and nucleic acids were studied. Techniques assessed included microfiltration, heat treatment and the extraction with phase systems formed by aliphatic alcohols. With 20% (w/w) primary lower phase it was found to be possible to separate 95% of the salt in 3–4 theoretical steps using counter‐current extraction.