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Immobilization of enzymes to porous‐bead polymers and silica gels activated by graft polymerization of 2,3‐epoxypropyl methacrylate
Author(s) -
Wójcik Anna,
Öobarzewski Jerzy,
BlaszczyłSka Teresa
Publication year - 1990
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280480305
Subject(s) - polymerization , bead , methacrylate , polymer , porosity , chemical engineering , materials science , polymer chemistry , chemistry , composite material , engineering
Three types of organic polymers and bead‐shape silica gels were activated by graft polymerization of 2,3‐epoxypropyl methacrylate; in some cases, epoxide groups on the support surface were modified to NH 2 groups. Eight active matrices so obtained were assessed as supports for immobilized enzymes using peroxidase, glucoamylase and urease. The immobilization yield of protein and specific activities of enzymes were better with supports containing NH 2 groups than with those containing epoxide spacer arms. Maximum enzyme immobilization and storage stabilities were obtained with silica‐gel beads activated by graft polymerization of 2,3‐epoxypropyl methacrylate. With all eight matrices tested, the immobilized enzymes showed good stability with not less than 82% of the original activity persisting after 28 days. The developed matrices have potential for use in process‐scale biotechnological operations.