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Biomass production by a thermotolerant yeast: Hansenula polymorpha
Author(s) -
Escalante Javier,
Caminal Gloria,
de Mas Carles
Publication year - 1990
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280480106
Subject(s) - biomass (ecology) , chemistry , fermentation , yield (engineering) , yeast , food science , hydrolysis , biochemistry , dry weight , chromatography , botany , biology , agronomy , materials science , metallurgy
Biomass production at high temperature by Hansenula polymorpha as part of a lignocellulosic utilizing process was studied. Compromise growth conditions (45°C and pH = 4.8) with an eventual saccharification step were established. The effects of stirring rate and initial glucose concentration on biomass yield coefficient, volumetric productivity and maximal cell density were determined. Process optimization led to a fed‐batch fermentation process: high yield (0.63 g dry cell g −1 glucose), volumetric productivity (1.3 g dry cell dm −3 h −1 ) and cell concentration (60 g dry cell dm −3 ) were obtained. At these conditions, significant arabitol excretion (18 g dm −3 ) as a unique by‐product associated with cell mass production was obtained, making more interesting a high temperature operating process.