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Comparison of oxygen supply methods for cultures of shear‐stress sensitive organisms including animal cell culture
Author(s) -
Mano Takashi,
Kimura Tatsuro,
Lijima Shinji,
Takahashi Katsuroku,
Takeuchi Hiroshi,
Kobayashi Takeshi
Publication year - 1990
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280470307
Subject(s) - aeration , oxygen , mass transfer , reynolds number , shear stress , bioreactor , chemistry , mass transfer coefficient , limiting oxygen concentration , chromatography , materials science , chemical engineering , analytical chemistry (journal) , thermodynamics , turbulence , composite material , physics , organic chemistry , engineering
In animal cell culture, oxygen supply sometimes limits cell growth. Therefore, four oxygen supply methods (free surface aeration, porous Teflon tubing, perfluorocarbon addition and external aerator) were compared in terms of oxygen transfer rate for suspension culture of animal cells. Oxygen transfer rate with the free surface aeration was dependent on the vertical position of the impeller and the stirred Reynolds number for h/H L >0.35, but no effect of the impeller position was observed for h/H L < 0.35. The relationship between the Reynolds and Sherwood numbers could be expressed by simple correlations. These correlations could also be applied to oxygen transfer using porous Teflon tubing. When oxygen was supplied with perfluorocarbon saturated with air, the overall mass transfer coefficient K L was estimated to be about 9.0 × 10 −3 cm s −1 , which was five‐fold greater than that of the surface aeration. Maximum cell densities which would be supported by four methods were calculated from values of K L a for different sizes of fermentor assuming that oxygen supply would be the rate‐limiting factor in animal cell culture.