Premium
Recovery of biosurfactants by ultrafiltration
Author(s) -
Mulligan Catherine N.,
Gibbs Bernard F.
Publication year - 1990
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280470104
Subject(s) - ultrafiltration (renal) , surfactin , rhamnolipid , membrane , pulmonary surfactant , chemistry , chromatography , micelle , critical micelle concentration , downstream processing , organic chemistry , bacteria , biochemistry , bacillus subtilis , aqueous solution , biology , pseudomonas aeruginosa , genetics
Ultrafiltration was used in a one‐step method to purify and concentrate biosurfactants—surfactin and rhamnolipids—from culture supernatant fluids. The ability of surfactant molecules to form micelles at concentrations above the critical micelle concentration allows these aggregates to be retained by relatively high molecular weight cut‐off membranes. Lower molecular weight impurities such as salts, free amino acids, peptides and small proteins are easily removed. Various molecular weight cut‐off membranes were examined for the retention of surfactin and rhamnolipids (mol. wts 1036 and 802 respectively). Amicon XM 50 was the superior membrane for retention of surfactin and a 160‐fold purification was rapidly achieved. The YM 10 membrane was the most appropriate for rhamnolipid recovery. Ultrafiltration can play an important role in biosurfactant purification as large volumes of media can be processed rapidly at extremely low cost.