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Determination of starch and maltose using immobilized amyloglucosidase and a glucose electrode in a flow injection system
Author(s) -
Gorton Lo,
Appelqvist Roger,
Johansson Gillis,
Scheller Frieder,
Kirstein Dieter
Publication year - 1989
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280460409
Subject(s) - maltose , glucose oxidase , starch , amylase , chemistry , chromatography , hydrolysis , steady state (chemistry) , biochemistry , biosensor , sucrose , enzyme , organic chemistry
Maltose and starch were hydrolysed to glucose by immobilized amyloglucosidase in a packed‐bed reactor and then measured by a glucose oxidase electrode in a flow injection analysis manifold. The steady‐state signals for both maltose and starch were constant for reactor residence times between 24 and 73 s. They were reduced on addition of mutarotase by c. 10 % with maltose and by c. 20 % with starch. The steady‐state value for glucose in the presence of mutarotase coincided with that for equivalent amounts of both maltose and starch according to Zulkowski, indicating a 100 % conversion im the amyloglucosidase reactor. For 40‐μm 3 samples, linear response curves were obtained over 100‐fold range of concentration. The maximum injection rate corresponded to 30 samples h −1 .