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Substrate sequestration and product inhibition in enzymic reactors: Analytical model and experimental studies
Author(s) -
Raul Avrom Z.,
Ilan Ehud,
Lotan Noah
Publication year - 1989
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280460205
Subject(s) - substrate (aquarium) , michaelis–menten kinetics , chemistry , product inhibition , kinetics , agarose , plug flow , chromatography , chemical engineering , enzyme , non competitive inhibition , biochemistry , enzyme assay , thermodynamics , biology , ecology , physics , quantum mechanics , engineering
The adverse influence that substrate sequestration exerts on the performance of a continuously operated, plug‐flow enzymic reactor was studied both experimentally and theoretically. Thus, glutathione‐S‐transferase was immobilized on activated agarose, and its performance in a packed‐bed reactor, both in the presence and in the absence of bovine serum albumin as sequestrator, was investigated. In parallel, an analytical model was developed, accounting for the behavior of systems which obey Michaelis—Menten kinetics, and which involve substrate sequestration and product inhibition. Substrate conversion in the reactor was found to decrease as sequestrator concentration and/or substrate—sequestrator association constant increased.