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Fractionation techniques in process biotechnology
Author(s) -
Scawen Michael D.,
Hammond Peter M.
Publication year - 1989
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280460202
Subject(s) - fractionation , process (computing) , size exclusion chromatography , chromatography , separation process , scale (ratio) , process engineering , filtration (mathematics) , process development , chemistry , separation method , protein purification , ion exchange , biochemical engineering , computer science , engineering , ion , mathematics , organic chemistry , enzyme , physics , statistics , quantum mechanics , operating system
The application of fractionation techniques to protein purification at a process or industrial scale is reviewed. The principles of various chromatographic separations are described and the choice and selection of available matrices discussed. Gel filtration, ion‐exchange hydrophobic interaction and affinity chromatographic techniques can all operate at process scale. HPLC is particularly suitable for process development and scale up without loss of efficiency. Magnetic separation, solvent partitioning and electrophoresis also offer potential for process scale operation. Recent developments also include improved matrices and the application of genetic engineering techniques to design proteins for efficient separation.