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Synthesis of 7‐amino‐4‐methylcoumarin (AMC) derivatives and their hydrolysis by plant cysteine proteinases
Author(s) -
Gray C. J.,
Sullivan J. M.
Publication year - 1989
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280460103
Subject(s) - papain , cysteine , chemistry , hydrolysis , bromelain , enzyme , amino acid , enzymatic hydrolysis , biochemistry , combinatorial chemistry
A number of derivatives of citrullyl‐7‐amino‐4‐methylcoumarin (Cit‐AMC) have been synthesised and characterised. Their hydrolysis, releasing the fluorescent 7‐amino‐4‐methylcoumarin, by the plant cysteine proteinases papain, ficin and bromelain was examined. Two derivatives in particular, Z‐Phe‐Cit‐AMC and Z‐Gly‐Phe‐Cit‐AMC, were shown to be good substrates for these enzymes. The possible use of the fluorogenic peptides in assays for these enzymes is discussed.