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Factors affecting biosurfactant production using Pseudomonas aeruginosa CFTR‐6 under submerged conditions
Author(s) -
Ramana K. Venkata,
Karanth N. G.
Publication year - 1989
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280450402
Subject(s) - glycolipid , chemistry , fermentation , nitrogen , yield (engineering) , food science , phosphate , pseudomonas aeruginosa , carbon fibers , bacteria , biomass (ecology) , pseudomonas , biochemistry , chromatography , biology , organic chemistry , agronomy , materials science , composite number , metallurgy , composite material , genetics
Studies on the biosurfactants produced by Pseudomonas aeruginosa CFTR‐6 revealed that they consisted of glycolipids R‐1 and R‐2. A time course study of fermentation indicated that the appearance of glycolipids in the fermentation broth coincided with the exhaustion of nitrogen and the commencement of the stationary phase with respect to biomass. The effect of variation of the media components such as carbon, nitrogen, phosphate and metal ions has been investigated. The following values were found to be optimum for biosurfactant production: glucose, 20 g dm −3 ; carbon to nitrogen ratio, 38; phosphate, 30 mmol dm −3 ; MgSO 4 .7H 2 O, 100 mg dm −3 . Addition of iron to the medium significantly diminished the glycolipid yield.