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Continuous enzymatic synthesis of Z ‐kyotorphin amide in an enzyme‐immobilized fixed‐bed reactor
Author(s) -
Clapés Pere,
MataAlvarez Joan,
Valencia Gregorio,
Reig Francesca,
Torres Josep L.,
GarcíaAntón JoséMaría
Publication year - 1989
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280450304
Subject(s) - amide , yield (engineering) , chemistry , immobilized enzyme , catalysis , dimethylformamide , factorial experiment , enzyme , hydrolysis , chymotrypsin , chromatography , organic chemistry , materials science , trypsin , solvent , mathematics , statistics , metallurgy
α‐Chymotrypsin immobilized on natural and inexpensive supports such as diatomaceous earth was used as catalyst for Z‐Tyr‐Arg‐NH 2 (Z‐kyotorphin amide) synthesis. In order to obtain the optimal reaction conditions, a 2 2 factorial experimental design was used. The factors considered were cosolvent ( dimethylformamide ) concentration and temperature; optimal product yield was achieved at 40% ( v/v ) dimethylformamide and 25°C. A sequential kinetic model was considered which generally gave good agreement between experimental and theoretical data for continuous synthesis of Z‐kyotorphin amide in a packed‐bed immobilized reactor system. The activation energy for the synthesis was determined to be 48.0 ± 2.3 kJ mol −1 .