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Application of ultrafiltration to the preparation of defined hydrolysates of bovine haemoglobin
Author(s) -
Piot JeanMarie,
Guillochon Didier,
Leconte Danielle,
Thomas Daniel
Publication year - 1988
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280420208
Subject(s) - hydrolysate , ultrafiltration (renal) , chromatography , chemistry , hydrolysis , pepsin , size exclusion chromatography , membrane , biochemistry , enzyme
Many uses of protein hydrolysates have been developed and applied to areas such as nutritional therapy, culture media, and the isolation of biologically active peptides. All these applications need carefully controlled and characterized hydrolysates. In order to produce such a type of hydrolysate, it is possible to use haemoglobin which is a very well defined and constant protein source. Enzymic hydrolysis of haemoglobin by pepsin was carried out at pilot‐plant scale in an ultrafiltration reactor with mineral membranes. The object was to obtain a reproducible, decolorized, salt‐free enzymic hydrolysate. Two types of membranes were tested having 10000 dalton (M5 type) and 20000 dalton (M4 type) cut‐offs. Little significant difference was observed in the final products when both types of membranes were used. Reproducibility of hydrolysates was verified by amino acid analysis and gel filtration chromatography. The haemoglobin hydrolysates produced contained more than 90% protein and are especially suitable for fine applications.