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Microscopic observation of Sudan Black B staining to monitor lipid production by microbes
Author(s) -
Thakur M. S.,
Prapulla S. G.,
Karanth N. G.
Publication year - 1988
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280420206
Subject(s) - sudan black b , staining , degree of unsaturation , stain , lipid droplet , food science , fermentation , chemistry , intracellular , biology , biochemistry , chromatography , genetics
In experimental investigations on the improvement of yields and productivities of lipids from oleaginous micro‐organisms in fermenters, a bottleneck is the speedy determination of the extent of accumulation of the intracellular lipids. The use of Sudan Black B for staining and direct observation under the microscope enables the rapid observation of the qualitative status of lipid production in the cells. A change in the colour of the stain from dark to light blue observed during the course of the fermentation has been found to be related to a change in the extent of unsaturation of the lipids.