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Kinetics of saccharification of raw starch by glucoamylase
Author(s) -
Matsumura Masatoshi,
Hirata June,
Ishii Satoshi,
Kobayashi Jiroh
Publication year - 1988
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280420107
Subject(s) - hydrolysis , chemistry , fermentation , starch , raw material , substrate (aquarium) , kinetics , enzyme , kinetic energy , enzymatic hydrolysis , ethanol , chromatography , food science , pulp and paper industry , biochemistry , organic chemistry , biology , ecology , physics , quantum mechanics , engineering
A kinetic model for saccharification of raw starch was derived by taking into account the structural features of glucoamylase. The kinetic parameters were determined by using the results of experiments on saccharification of raw sweet potato starch, which were carried out at various substrate and enzyme concentrations in a temperature range from 23 to 40°C. From analysis of the experimental results it was concluded that the adsorption of enzyme was strongly dependent on temperature, and that two subsites took part in glucose inhibition, which was more pronounced at low temperature. The proposed kinetic model successfully predicted the progress of saccharification up to a high conversion, and also the progress of ethanol production in a simultaneous saccharification and fermentation.