The automated production by immunoaffinity chromatography of the human pituitary glycoprotein hormones thyrotropin, follitropin and lutropin | Zendy

Jack George W. | Zendy; Blazek Richard | Zendy; James Keith | Zendy; Boyd Janice E. | Zendy; Micklem Lisel R. | Zendy

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The automated production by immunoaffinity chromatography of the human pituitary glycoprotein hormones thyrotropin, follitropin and lutropin

Author(s) -

Jack George W.,

Blazek Richard,

James Keith,

Boyd Janice E.,

Micklem Lisel R.

Publication year - 1987

Publication title -

journal of chemical technology and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.64

H-Index - 117

eISSN - 1097-4660

pISSN - 0268-2575

DOI - 10.1002/jctb.280390107

Subject(s) - glycoprotein , hormone , monoclonal antibody , affinity chromatography , chemistry , chromatography , biological activity , medicine , endocrinology , biology , antibody , biochemistry , in vitro , immunology , enzyme

Human pituitary TSH, FSH and LH were purified from a side fraction of human pituitary growth hormone production by immunoaffinity chromatography on immobilised hormone‐specific monoclonal antibodies. Each hormone was obtained in biologically active form and was substantially free, <0·5% by weight, of the other glycoprotein hormones. The method was automated using a time‐based programmer and by applying the unbound fraction from one purification to columns of different specificities each of the three hormones was obtained from a single batch of starting material.

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