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The automated production by immunoaffinity chromatography of the human pituitary glycoprotein hormones thyrotropin, follitropin and lutropin
Author(s) -
Jack George W.,
Blazek Richard,
James Keith,
Boyd Janice E.,
Micklem Lisel R.
Publication year - 1987
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280390107
Subject(s) - glycoprotein , hormone , monoclonal antibody , affinity chromatography , chemistry , chromatography , biological activity , medicine , endocrinology , biology , antibody , biochemistry , in vitro , immunology , enzyme
Human pituitary TSH, FSH and LH were purified from a side fraction of human pituitary growth hormone production by immunoaffinity chromatography on immobilised hormone‐specific monoclonal antibodies. Each hormone was obtained in biologically active form and was substantially free, <0·5% by weight, of the other glycoprotein hormones. The method was automated using a time‐based programmer and by applying the unbound fraction from one purification to columns of different specificities each of the three hormones was obtained from a single batch of starting material.