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Effects of nutrients on α‐amyiase production and plasmid stability in fed‐batch culture of recombinant bacteria
Author(s) -
Iijima Shinji,
Kai Kenichi,
Mizutani Satoru,
Kobayashi Takeshi
Publication year - 1986
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280361106
Subject(s) - plasmid , bacteria , escherichia coli , industrial fermentation , fed batch culture , recombinant dna , microbiology and biotechnology , nutrient , biology , bacterial growth , food science , plasmid preparation , chemistry , biochemistry , fermentation , gene , ecology , genetics , pbr322
Escherichia coli harbouring a recombinant plasmid containing the α‐amylase gene (HB101/pHI301) and its derivative which can stably maintain the plasmid (HB101/pHI301A) were cultivated in a mini‐jar fermenter. By controlling dissolved oxygen (DO) at a concentration of 2–3 μg/l and supplementing with suitable amounts of nutrients, the organisms could grow to a concentration (up to 40 g dry cell l −1 ) in a semi‐synthetic casamino acid medium. During culture the plasmid was maintained stably in both strains. In a synthetic medium, both micro‐organisms grew less well and plasmid‐free segregants were observed with HB101/pHI301 during the fed‐batch culture. On the other hand, plasmid pHI301A was stably maintained throughout the fed‐batch culture of HB101/pHI301A. Growth inhibitors, which may be metabolic products, accumulated in the medium and reduced bacterial growth.