Premium
The use of the F.p.l.c. R system in method development and process monitoring for industrial protein chromatography
Author(s) -
Low D. K. R.
Publication year - 1986
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280360802
Subject(s) - chromatography , process (computing) , process development , process engineering , chemistry , computer science , engineering , operating system
The f.p.l.c. R for high performance biomolecular purification is now widely used in laboratory and pilot scale separations, both as a means of developing new methods for purification and for analysing the purity of intermediary and final products. The speed of the technique means that it is possible to analyse fractions from a process step and to receive the results within 15–30 min. Two aspects are examined in this paper: the monitoring of protein product formation during fermentation and the analysis of fractions in large scale chromatography. The suitability of the technique in an operating process is discussed.