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Design of immobilised glucoamylase reactors using a simple kinetic model for the hydrolysis of starch
Author(s) -
Cabral J. M. S.,
Novais J. M.,
Cardoso J. P.,
Kennedy J. F.
Publication year - 1986
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.280360602
Subject(s) - hydrolysis , starch , chemistry , continuous stirred tank reactor , chromatography , kinetic energy , immobilized enzyme , dispersion (optics) , packed bed , continuous reactor , corn starch , chemical engineering , catalysis , enzyme , organic chemistry , physics , quantum mechanics , optics , engineering
A comparative study is reported of the three main types of continuous reactors, continuous feed stirred tank reactor (CFSTR), packed bed reactor (PBR) and fluidised bed reactor (FBR), for the conversion at 50°C of a 30% (w/w) corn syrup 40 DE using glucoamylase (exo‐1,4‐D‐glucosidase, EC 3.2.1.3) immobilised on carbonyl derivatives of titanium(IV)‐activated porous silica by a method developed previously. 1–3 The hydrolysis of starch in these reactors is described by a simple kinetic model 4 which involves the intrinsic kinetic constants as well as mass transfer and dispersion effects, and allows the computation of enzyme activity values under continuous operation. FBR appears to be more effective for the hydrolysis of starch. This reactor also confers a better operational stability (t 1/2 =775h) on the immobilised enzyme than the other immobilised glucoamylase reactors (PBR, t 1/2 =629h; CFSTR, t 1/2 =239h).